Hi everyone
As some of you know I am working on a Frustrated TIR device. Following the lead of fellow microscopists I built a sort of transmitted darkfield (Fr-TIR) fluorescence illumination system. Following see the results.
The sample is Pinus XS male strolibus with microspore. Mounted on slide.
I used the setup seen in the image. Bottom to top we have the light guide the sample and the yellow filter. For this one I used a very simple light guide with a Blue LED strip 452-455nm.
FrTIR Fluoresccence.jpg
Fr-TIR with BLUE LED 452-455nm and Yellow filter LPF
5x 1 c small.jpg
Brightfield
5x 1 bis 2 small.jpg
Fr-TIR device white light
5x 1 small.jpg
RGDS
Ramon