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Foren => Mikroskopie-Forum => Thema gestartet von: Changhee in März 16, 2023, 15:30:10 NACHMITTAGS

Titel: How do I upgrade resolution for jenalumar microscope?
Beitrag von: Changhee in März 16, 2023, 15:30:10 NACHMITTAGS
Dear all.

I would like to try to upgrade the resolution of the jenalumar microscope.
So, I would like to upgrade the condenser lens (apl 0.9) to apl 1.4.
We are also thinking of making and combining capacitors or adapters that can be mounted immediately.
Also, I want to know if the resolution can be increased in that way and how it affects the image quality.

(https://www.mikroskopie-forum.de/pictures013/339969_36849274.jpg)


Thank you for your answer and Best regards,
Changhee
Titel: Re: How do I upgrade resolution for jenalumar microscope?
Beitrag von: Apochromat in März 17, 2023, 20:16:47 NACHMITTAGS
Dear Changhee,

you need the condenser front lens Achr. 1,30 (304332:032.24/6) or Achr. 1,3 pol together with its matching rump optics (see right image and the arrows, green is interchangeable, red not): The rump optics of the condenser head achr. apl. 0,9 does not work together with the higher aperture front lenses! They came with their own rump optics. Sometimes, condenser front heads are offered separetely from their rump by people who are not aware of that. Such purchases are useless. Also, the early JENAVAL condensers had different threads on head and rump compared to what was offered later. The versions from 1982 - 1984 had even female and male threads reversed from the later versions! They have an engraving in white (not the silk- screen print in silver colour).

So You need this: The front and rump optics shown, are the latest versions discernible by the silver coloured silk- screen print of "Achr. 1,3" or "Achr. 1,3 pol", made until 1990. Depicted here:

(https://www.mikroskopie-forum.de/pictures013/340023_36849274.jpg)



Regarding the image quality: In phase contrast, the higher aperture condenser front optics Achr. 1,30 (used with self- made, modified Ph- ring stops) just gives a brighter image (factor ~ 1,8x) not much better resolved -if at all:


(https://www.mikroskopie-forum.de/pictures013/340023_63208015.jpg)

Marine Diatom (J. LIEDER; Mr. Heinz Fegert lege artis). JENA 250-CF Apochromat HI 100x/1,40 Phv oo/0,17-A with phase contrast and condenser head Achr. apl. 0,9 (Colour image with LED- light source made by Stephan Hiller, no image manipulation/ processing after acquisition)


In DIC there is a difference, but that would be a very lenghty story to explain here, especially what is all needed to make it work. And still, I am not sure, if it is worth all the effort ... The condenser head with N.A. 0,9 is also more than excellent for almost everything I could think of (except a few diatoms here).

(https://www.mikroskopie-forum.de/pictures013/340023_32001227.jpg)

Freshwater nano-flagellate Bodo angustus. Length is approx. 15 µm. JENA 250-CF Apochromat HI 100x/1,40 oo/0,17-A with DIC and condenser head Achr. 1,3 pol immersed with IMMERSOL 518 F (Colour image with PANASONIC LUMIX GX-9 and PANASONIC TTL DMW-FL 580 used as micro flash and made by Jürgen Stahlschmidt, no image manipulation/ processing after acquisition)



If You cannot access the JENAVAL Achr. 1,3 front and rump optics, a home made adapter for the much easier to get CARL ZEISS, Oberkochen condenser achr. apl. 1,40 H, DIC will also work beautiful with such a condenser on the JENAVAL in brightfield or DIC. As I have no such adapter any longer (its gone since more than 25 years), I cannot depict it for You here ...




Regards

Michael
Titel: Re: How do I upgrade resolution for jenalumar microscope?
Beitrag von: Changhee in März 18, 2023, 15:39:10 NACHMITTAGS
Dear Michael,

Thank you for your help.
I'm looking for arch 1.3 condensor lens according to your advise. But. It is so difficult.

Best regards,
Changhee.pyeoun
Titel: Re: How do I upgrade resolution for jenalumar microscope?
Beitrag von: Changhee in März 18, 2023, 17:19:55 NACHMITTAGS
Dear Michael,

I found the website and sent an email, but I received a return mail saying that I couldn't find the domain. I'm frustrated because I can't make a phone call because I live in Korea.

https://www.mikroskop-online.de/Verkauf%20Mikroskopteile.htm

info@microscopes-online.de

Best regards,
Changhee.pyeoun
Titel: Re: How do I upgrade resolution for jenalumar microscope?
Beitrag von: Apochromat in März 18, 2023, 17:54:19 NACHMITTAGS
Dear Changhee,

the JENAVAL condenser heads from that dealer are all long gone, unfortunately. But the correct contact e-mail is: info@mikroskope-online.de

And as said, the JENAVAL 250- CF DIK/ DIC does not work with the official components any longer with the JENAVAL Achr. 1,3 condenser head.

But if you have a black condenser carrier Z from CZ Oberkochen and the fitting Satzkondensor 0,32 (465267) with the achr. apl. 1,4 Pol front lens (465268) and a matching DIC- prism (e.g. condenser DIC- prism 434405, N.A. >/= 0,5; only one prism fits into this condenser at a time) and somebody makes an adapter for the female CZO condenser carrier Z- dovetail on the JENAVAL condenser carrier- dovetail, that works, as these condenser DIC- prisms are optimized for that condenser N.A. regarding their crystallography. You only have to rotate that condenser until the condenser prism orientation/ azimuth is in that unusual position, where You can detect a horizontal interference stripe (condenser prism only, without objective- sided prism engaged):


(https://www.mikroskopie-forum.de/pictures013/340052_41962596.jpg)

The current CZ reflected light polarizer for the Axiovert 5 MAT or the current Axioscope 5/7 models (427710-9000) also fits to the JENAVAL as analyzer rotatable by 90° and You will need a separate, simple CZ polarizer 453600 to insert into the light exit opening (the de Senarmont DIK polarizer is not used then).

Sorry for that

Michael
Titel: Re: How do I upgrade resolution for jenalumar microscope?
Beitrag von: Changhee in April 05, 2023, 14:18:18 NACHMITTAGS
Dear Michael,

I have a question. I have a jenalumar with a DIK function.However, the DIK Apo 12.5 KT tube filter among drum filters cannot be used due to balsam contamination. So, I found a product of DIK 12.5/25 KT on eBay. I would like to ask you if you know the difference between Apo and not.

< Ebay >


<My DIK filter tube>
(https://www.mikroskopie-forum.de/pictures013/340866_63208015.jpg)

Best Regards,
Changhee.pyeoun
Titel: Re: How do I upgrade resolution for jenalumar microscope?
Beitrag von: Apochromat in April 06, 2023, 19:59:48 NACHMITTAGS
Dear Changhee,

the prism "DIK PApo 25" for the GF- PApo 25x/0,65 oo/0,17-A is the same as the prism "DIK Apo 12,5" for the famous Apo 12,5x! So You can use the prism DIK PApo 25 for both objectives equally well. It contained the same prism type 305.24.

The prism "DIK 12,5/25" works also for the GF- PApo 25x (but is lightly less homogeneous, but with perfect image shear) but not for the Apo 12,5x.

Thanks

Michael
Titel: Re: How do I upgrade resolution for jenalumar microscope?
Beitrag von: Changhee in April 07, 2023, 14:33:19 NACHMITTAGS
Dear Michael,

Thank you for your help.

I will focus on upgrading the condenser according to your advice.
So, I am looking hard on eBay. :(
Thank you again for your help.

Best regards,
Changhee.pyeoun
Titel: Re: How do I upgrade resolution for jenalumar microscope?
Beitrag von: Apochromat in April 07, 2023, 19:36:22 NACHMITTAGS
Dear Changhee,

normally I do not refer to ebay auctions, but the modulator disk prism for the JENAVAL contrast that You need for the Apo 12,5x objective is also momentarily available from that source:

(https://www.mikroskopie-forum.de/pictures013/340966_41962596.jpg)

Good luck!

Michael
Titel: Re: How do I upgrade resolution for jenalumar microscope?
Beitrag von: Changhee in April 09, 2023, 15:58:54 NACHMITTAGS
Dear Michael,

Thank you for your answer.

I have DIK Papo 25 KT and DIK Fl 50/100 Papo KT.
Recently, I applied a new phase contrast ring and  PA-Phv Set 25x setting, but, the DIK setting went wrong, so I'm having a hard time.
Is there an easy way to match the DIK setting?

Best Regards,
Changhee.pyeoun
Titel: Re: How do I upgrade resolution for jenalumar microscope?
Beitrag von: Apochromat in April 09, 2023, 19:44:37 NACHMITTAGS
Dear Changhee,

I do not quite understand what your difficulty is. But if You do not mind, we can comminucate via PN. I will send You one.

Thanks

Michael
Titel: Re: How do I upgrade resolution for jenalumar microscope?
Beitrag von: Changhee in Mai 21, 2023, 08:11:41 VORMITTAG
Dear Michael,

I have a question regarding DIK (Digital Image Keying) setup adjustments.
The attached image shows the DIK settings I'm using, and I have applied the same method for all magnifications.
The DIK setting image looks very different between the 50x and 25x settings. I'm curious about the reason for this and whether it's appropriate. Also, I'm considering purchasing the DIK PApo 12.5KT filter because the DIK characteristics are not visible with the current DIK filter.
Is there a way to achieve similar results with the DIK PApo 25KT filter?
I lack specialized knowledge about microscopes and haven't received guidance on the optimal settings from jenalumar, so I don't know the criteria.
By the way, in the attached images, the drum filter in Image 3 is adjusted forward for rotation, and this is associated with the condenser lens and wheel filter settings. However, to use PHV lens and filter, it requires adjusting the condenser lens, so ultimately both need to be adjusted to use PHV.
I am not received any information from PM yet, so I will send you with my email address. (changhee.pyeoun@gmail.com)


Best regards,
changhee
Titel: Re: How do I upgrade resolution for jenalumar microscope?
Beitrag von: Apochromat in Mai 21, 2023, 22:06:15 NACHMITTAGS
Dear Changhee,

I have just resent my PN from a few weeks ago to you.

All the best

Michael