The cuticle of Paramecium!

arturoag75 · September 13, 2011, 18:43:44 NACHMITTAGS

Hi,
take a look this paramecium showing the gridded cuticle!
obb. 40x Phase and DIC. canon 600d!
curiosly this paramecium did not explode like others under coverglass!
best
Arturo

Florian Stellmacher · September 13, 2011, 20:18:25 NACHMITTAGS

Buona sera, Arturo,

really nice photographs! Is this the "old" Zeiss DIK for the Photomikroskop II or the newer DIK for the Photomikroskop III?

I have the older version and my results are fare from yours.

Is there a special trick?

Ciao,
Florian

arturoag75 · September 14, 2011, 08:39:24 VORMITTAG

Hi Florian,
i got the third version of DIC whit single prism for each objective you can see im my website!
there is no trick only a little settage of level in Photoshop!
many thanks for comment!
Arturo

Florian Stellmacher · September 14, 2011, 10:30:52 VORMITTAG

Bon giorno, Arturo,

thank you. You are using a setup different from mine, and you have the right touch in using Photoshop.

By the way: If you do not merge two photographs within the same file the photographs will be displayed in larger size in this forum - this would look even better!

Ciao,
Florian

arturoag75 · September 14, 2011, 15:54:10 NACHMITTAGS

..thanks to you Florian!
arturo

Peter V. · September 14, 2011, 21:22:26 NACHMITTAGS

Hello Arturo,

what is this

I have never seen such structures on the surface of Paramecium before. Is it typical for all Paramecia or is this a chracteristic mark of the speical kind of Paramecium?

Regards
Peter

arturoag75 · September 15, 2011, 21:30:56 NACHMITTAGS

Hi Peter,
i think this is a structure of all genera of paramecium!
clic on the link below and scroll the page you find an image trought SEM microscope and a diagram!
best
Arturo

http://www.sfu.ca/~fankbone/v/lab02.html

rekuwi · September 16, 2011, 21:50:47 NACHMITTAGS

Lieber Arturo,

sehr schöne Fotos und ein toller Link! Danke.

Liebe Grüße
Regi

arturoag75 · September 17, 2011, 10:53:18 VORMITTAG

many thanks

Arturo

Bernd · September 17, 2011, 13:59:32 NACHMITTAGS

Hi Arturo,

I was puzzeled by your Paramecium photo when I saw it the first time and I am still puzzeled now, a few days later. For me, it looks as if it is something "wrong" with this critter. Paramecium has huge numbers of cilia all over its body which should obscure the structure of the pellicula. And I never managed to see clearly anything but moving cilia when I focused on the surface of the cell. Anyway, the posted image is rather small. Can you post a larger version of your DIC image so that the presence or absence of cilia can be seen more clearly? Maybe, you can also add a picture showing the micro- and macronucleus?
The link above in my opinion shows a Paramecium which has been deciliated before imaging to show the cuticular structure.

Excellent image, anyway
Bernd

arturoag75 · September 17, 2011, 18:29:41 NACHMITTAGS

Hi here other shots...unfortunately i've not a shot of macro and micro nucleus but i can post (last two pics) anoter one present in that drop of water!
hope this help you
best
arturo

[imghttp://img90.imageshack.us/img90/8226/img1924kr.jpg]https://www.mikroskopie-forum.de/pictures007/73244_6494098.jpg[/img]

[imghttp://img854.imageshack.us/img854/658/img1912xk.jpg]https://www.mikroskopie-forum.de/pictures007/73244_64931457.jpg[/img]

hope this help
best
arturo