Micrasterias

[Jan l'Amie]

Micrasterias thomasiana


Micrasterias rotata


Micrasterias papillifera


Micrasterias truncata


Micrasterias jenneri


Micrasterias crux melitensis


Micrasterias americana (only one found ever)

[koestlfr]

Hallo Jan!

I like your pictures, but I would suggest to make a white-balance and to increase the contrast a little bit! I suppose that you use a DIC.

Can you tell us a little bit about the technique, objectives .......

Kind regards
Franz

[Jan l'Amie]

Those are pictures from older series, this is a more resent one:



I use no DIC but Do it yourself made oblique light and Rheinberg filters.



The microscoop is a Byomic BYO 500T Trinoculair with PL objectives and a 2,5x photo-oculair.
The camera is a Canon 50D.

[rhamvossen]

Hallo,

Nice DIC-like pictures you got with oblique illumination. I also have a few "Byomic" objectives, they are Chinese no-name achromats from a Dutch importer and their quality is quite good, except for the fact that they give a bit of a yellowish background in the image. You need an extra daylight filter to correct for that. The sharpness in some of your images is not 100% spot-on, I assume that it's the camera? Best regards,

Rolf

[Jan l'Amie]

My PL objectives can compete with much more expensive objectives
of other brands and I'm very satisfied with them.
The DIC effect of an oblique light filter is not as from an very expensive
DIC microscope and a desmid is not a realy flat object, so if it is not
100 % horizontal unther the microscope you can get some unsharpness.
In that case you can better make some stacking pictures.

Stack of five frames: