Mitotic spindle - holophryid ciliate

[Ariel Qassis]

Hello,
here is my first recording of a ciliate mitotic spindle,
enjoy

[tlansing]

Hi Ariel,

Very cool images!

Best wishes,
Tim

[Jürgen Boschert]

Hello Ariel,

just outstanding, thanks!

[Spectrum]

Hello Ariel,
Pushing the boundaries even farther.🤩
What's next?
Greetings Holger

[Ariel Qassis]

Danke, Tim, Jürgen und Holger!

[anne]

Dear Ariel,
I have Never Seen this before!
Thanks for Sharing, absolutely great.
Best
Anne

[mikrowastl]

great - thank you Ariel

[Ariel Qassis]

Danke, Anne und Hans!

[Wes]

Hi Ariel,

This is a remarkable observation. As far as I know ciliate macronuclei divide amitotically without distinct phases and chromosomes being visible. If possible try to record the completion of the division process and perhaps try to get in contact with someone who is involved with ciliates in a professional academic manner to evaluate these observations. Once again, well done for this extraordinary documentation.

Would you mind telling us the technical specs of your documentation? What objectives etc. were used?

Best regards,
Wes

[Spectrum]

Hello Wes, hello Ariel,
There are exceptions:
https://link.springer.com/article/10.1007/BF00283410
Greetings Holger

[rlu]

Hello Ariel,

great photo, observation!

The internet must be full of such photos, but it isn't - strange enough in relation to ciliate and other protists.

I recently came across some questions about what happens to the rest of the cell during mitosis and how the other cell organelles are replicated.

One part is the nucleoli. 'Das Kernkörperchen' seems to replicate with bubbles/vesicles instead of the mitotic spindle. Unfortunately, I don't know enough about it.

It's a really interesting topic and observation, and I hope there will be more content.

Merci
Rudolf

[Ariel Qassis]

Wes, Holger, und Rudolf-
Thank you for your insights.
Although the MA is usualy divides amitoticaly, I believe this one is the MI, which is known to divide mitotically.
These holophryids got an MA of over 50µm
If it's not the MI, then I have a wild theory-
Those holophryids in my sample are severely infected by probably microsporidia (video in my channel), which are known to cause a wide range of strange genetic phenomena
Anyway, Im not an expert on the subject, and couldnt find another one yet, but I keep looking.