Was benötigt man, um ein Zeiss Standard auf DIC/DIK umzurüsten?

[JB]

Hi there,

I think René is right on this one. The objective will not change from NA 1.4 to 0.99; is stays at 1.4.

When using an immersion condenser NA=1.4 without immersion, the aperture will be somewhere between 0.8 and 0.9 (depends on the model; hard to predict because the condenser will usually not be corrected for both modes, but not 0.99).

When using an objective NA=1.4 with a condenser NA=1.4, the maximum resolution that can be achived is 240 nm (for green light). When the same objective is used with the dry condenser NA=0.9, the resolution will be 270 nm; at NA=0.8, the resolution is 277 nm.

The calculations are from this publication:
Baker (1952): Remarks on the Effect of the Aperture of the Condenser on Resolution by the Microscope. Quarterly Journal of Microscopical Science 93: 375-377.

Kind regards,

Jon

[novus]

Hello René!

As an aside, in DIC mode, the effect is less because of the full aperture lighting that is generally employed: the condenser iris is not closed to near the phase ring.

Well, that's good to hear. However, even if phase rings will not lead to any distortions, I guess that it would be still necessary to use Plan-objectives in DIC mode, isn't it?

kind regards,
Michael

[Rene]

Hello Michael,

Well, the old Zeiss DIC was calculated to be used with planachro's, but there are no principal reasons for that.

Good luck hunting, René

[novus]

Dear all: BINGO 

Best wishes,
Michael

[Peter V.]

Hallo Michael,

Herzlichen Glückwunsch!!!

Und? Hab' ich gut geschätzt?  

Herzliche Grüße
Peter

[novus]

Peter, Du bist ein Fuchs

Herzliche Grüße,
Michael

[Eckhard]

All,

NA_total = (NA_objective + NA_condensor) / 2

Best regards
Eckhard